肉桂醛
化学
色谱法
质谱法
药代动力学
气相色谱-质谱法
选择性离子监测
分析物
肉桂醇
检出限
气相色谱法
校准曲线
药理学
有机化学
医学
催化作用
作者
Hang Zhao,Yanhua Xie,Qian Yang,Yu Cao,Honghai Tu,Wei Cao,Siwang Wang
标识
DOI:10.1016/j.jpba.2013.10.044
摘要
A selective and sensitive method utilizing gas chromatography–mass spectrometry was developed for simultaneous determination of cinnamaldehyde, cinnamyl alcohol, and methyl cinnamate in rat plasma. Cinnamaldehyde and cinnamyl alcohol can inter-convert to one another in rats, thus simultaneous quantifying both analytes provided a reliable and accurate method of assessment. Three qualifying ions (131 m/z, 105 m/z and 92 m/z) were chosen for simultaneous quantification of cinnamaldehyde and its metabolites. In this study, the calibration curves demonstrated a good linearity and reproducibility over the range of 20–2000 ng/ml (r2 ≥ 0.999) for all analytes. Furthermore, the sensitivity of gas chromatography–mass spectrometry revealed sufficient lower limit of quantitation and detection of 20 ng/ml and 5 ng/ml, respectively, in the pharmacokinetic analysis. The intra- and inter-day precision variations were less than 10.4% and 12.2%, respectively, whilst accuracy values ranged from −8.6% to 14.8%. All analytes were stable in plasma and in processed samples at room temperature for 24 h with no significant degradation after three freeze/thaw cycles. A small amount of the administered cinnamaldehyde had long half-life of 6.7 ± 1.5 h. In this study, gas chromatography–mass spectrometry was demonstrated to be a powerful tool for the pharmacokinetic studies of rats after intravenous and oral administration of cinnamaldehyde.
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