生物转化
融合蛋白
细胞
噬菌体展示
细胞融合
表面蛋白
肽库
融合
肽
化学
细胞生物学
生物
生物化学
重组DNA
肽序列
基因
哲学
病毒学
发酵
语言学
作者
Sang Yup Lee,Jong Hyun Choi,Zhaohui Xu
标识
DOI:10.1016/s0167-7799(02)00006-9
摘要
Cell-surface display allows peptides and proteins to be displayed on the surface of microbial cells by fusing them with the anchoring motifs. The protein to be displayed - the passenger protein - can be fused to an anchoring motif - the carrier protein - by N-terminal fusion, C-terminal fusion or sandwich fusion. The characteristics of carrier protein, passenger protein and host cell, and fusion method all affect the efficiency of surface display of proteins. Microbial cell-surface display has many potential applications, including live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption.
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