Identification of Hydrophobic Tags for the Degradation of Stabilized Proteins

ChemBioChemVolume 13, Issue 4 p. 538-541 Communication Identification of Hydrophobic Tags for the Degradation of Stabilized Proteins Dr. Hyun Seop Tae, Dr. Hyun Seop Tae Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA) These authors contributed equally to this work.Search for more papers by this authorDr. Thomas B. Sundberg, Dr. Thomas B. Sundberg Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA) These authors contributed equally to this work.Search for more papers by this authorDr. Taavi K. Neklesa, Dr. Taavi K. Neklesa Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA) These authors contributed equally to this work.Search for more papers by this authorDevin J. Noblin, Devin J. Noblin Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Search for more papers by this authorDr. Jeffrey L. Gustafson, Dr. Jeffrey L. Gustafson Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Search for more papers by this authorDr. Anke G. Roth, Dr. Anke G. Roth Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Search for more papers by this authorKanak Raina, Kanak Raina Department of Chemistry, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Search for more papers by this authorProf. Craig M. Crews, Corresponding Author Prof. Craig M. Crews [email protected] Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA) Department of Chemistry, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA) Department of Pharmacology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Search for more papers by this author Dr. Hyun Seop Tae, Dr. Hyun Seop Tae Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA) These authors contributed equally to this work.Search for more papers by this authorDr. Thomas B. Sundberg, Dr. Thomas B. Sundberg Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA) These authors contributed equally to this work.Search for more papers by this authorDr. Taavi K. Neklesa, Dr. Taavi K. Neklesa Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA) These authors contributed equally to this work.Search for more papers by this authorDevin J. Noblin, Devin J. Noblin Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Search for more papers by this authorDr. Jeffrey L. Gustafson, Dr. Jeffrey L. Gustafson Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Search for more papers by this authorDr. Anke G. Roth, Dr. Anke G. Roth Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Search for more papers by this authorKanak Raina, Kanak Raina Department of Chemistry, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Search for more papers by this authorProf. Craig M. Crews, Corresponding Author Prof. Craig M. Crews [email protected] Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA) Department of Chemistry, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA) Department of Pharmacology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511 (USA)Search for more papers by this author First published: 23 January 2012 https://doi.org/10.1002/cbic.201100793Citations: 59 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract New HyTs are a knockout: We previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36. Citing Literature Supporting Information Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description cbic_201100793_sm_miscellaneous_information.pdf913.5 KB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume13, Issue4March 5, 2012Pages 538-541 RelatedInformation