氧化应激
酒精性肝病
DNA损伤
病理
酒精性肝炎
胆管
肝硬化
肝炎
慢性肝病
肝病
炎症
医学
生物
DNA
内科学
生物化学
作者
Takuya Kitada,Shuichi Seki,Shuji Iwai,Takao Yamada,Hiroki Sakaguchi,Kenichi Wakasa
标识
DOI:10.1016/s0168-8278(01)00171-4
摘要
Background/Aims: 8-Hydroxydeoxyguanosine (8-OHdG) is a promutagenic DNA lesion produced by oxygen radicals and is recognized as a useful marker in estimating DNA damage induced by oxidative stress. Methods: Hepatic expression of 8-OHdG was immunohistochemically investigated in control and diseased human livers. Results: While no positive immunolabeling for 8-OHdG was observed in control livers, 8-OHdG was widely evident in diseased livers. Nuclear expression of 8-OHdG in the hepatocytes and bile duct cells were found in various forms of chronic hepatitis. 8-OHdG-positive hepatocytes were especially abundant in the periportal area with piecemeal necrosis and prominent cell infiltration. The number of positive hepatocytes significantly increased with the progression of severity of chronic hepatitis activity (rs=0.68, P<0.05). In alcoholic liver disease, nuclear expression of 8-OHdG was detected in the hepatocytes in the area of alcoholic hepatitis. Regarding primary biliary cirrhosis, 8-OHdG was preferentially detected in the nuclei of injured bile ducts (11 of 12 cases, 91.7%) and occasionally (2 of 12 cases, 16.7%) in the nuclei of hepatocytes around the bile duct lesions. Conclusions: These results indicate that oxidative DNA damage is common in various forms of chronic liver disease suggesting a possible link between chronic inflammation and hepatocarcinogenesis.
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