硫酸化
化学
动力学(音乐)
核磁共振波谱
立体化学
生物化学
物理
声学
作者
Ismael N L Queiroz,Xiaocong Wang,John Glushka,Gustavo RC Santos,Ana Paula Valente,James H. Prestegard,Robert J. Woods,Paulo A.S. Mourão,Vitor H. Pomin
出处
期刊:Glycobiology
[Oxford University Press]
日期:2014-12-19
卷期号:25 (5): 535-547
被引量:21
标识
DOI:10.1093/glycob/cwu184
摘要
Sulfated fucans from sea urchin egg jelly express well-defined chemical structures that vary with species. This species specificity regulates the sperm acrosome reaction, a critical step to assure intra-specific fertilization. In addition, these polysaccharides are involved in other biological activities such as anticoagulation. Although sulfation patterns are relevant to the levels of response in both activities, conformation and dynamics of these glycans are also contributing factors. However, data about these features of sulfated fucans are very rare. To address this, we have employed nuclear magnetic resonance experiments combined with molecular dynamics on structurally defined oligosaccharides derived from two sulfated fucans. The results have indicated that the oligosaccharides are flexible in solution. Ring conformation of their composing units displays just the 1C4 chair configuration. In a particular octasaccharide, composed of two tetrasaccharide sequences, inter-residual hydrogen bonds play a role to decrease dynamics in these repeating units. Conversely, the linking disaccharide [-3)-α-l-Fucp-2(OSO3−)-(1–3)-α-l-Fucp-4(OSO3−)-(1-] located right between the two tetrasaccharide units has amplified motions suggested to be promoted by electrostatic repulsion of sulfates on opposite sides of the central glycosidic bond. This conjunction of information about conformation and dynamics of sulfated fucan oligosaccharides provides new insights to explain how these glycans behave free in solution and influenced by sulfation patterns. It may also serve for future studies concerning structure–function relationship of sulfated fucans, especially those involving sea urchin fertilization and anticoagulation.
科研通智能强力驱动
Strongly Powered by AbleSci AI