咖啡因
色谱法
化学
儿茶素
高效液相色谱法
没食子酸表没食子酸酯
萃取(化学)
没食子酸
表儿茶素没食子酸盐
检出限
绿茶
洗脱
固相萃取
溶剂
多酚
食品科学
核化学
生物化学
抗氧化剂
内分泌学
医学
作者
Afidah Abdul Rahim,S. Nofrizal,Bahruddin Saad
标识
DOI:10.1016/j.foodchem.2013.09.131
摘要
A rapid reversed-phase high performance liquid chromatographic method using a monolithic column for the determination of eight catechin monomers and caffeine was developed. Using a mobile phase of water:acetonitrile:methanol (83:6:11) at a flow rate of 1.4 mL min−1, the catechins and caffeine were isocratically separated in about 7 min. The limits of detection and quantification were in the range of 0.11–0.29 and 0.33–0.87 mg L−1, respectively. Satisfactory recoveries were obtained (94.2–105.2 ± 1.8%) for all samples when spiked at three concentrations (5, 40 and 70 mg L−1). In combination with microwave-assisted extraction (MAE), the method was applied to the determination of the catechins and caffeine in eleven tea samples (6 green, 3 black and 2 oolong teas). Relatively high levels of caffeine were found in black tea, but higher levels of the catechins, especially epigallocatechin gallate (EGCG) were found in green teas.
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