Phenolic Acid–Amino Acid Adducts Exert Distinct Immunomodulatory Effects in Macrophages Compared to Parent Phenolic Acids

咖啡酸 化学 活性氧 生物化学 肿瘤坏死因子α 抗氧化剂 生物 内分泌学
作者
Jingyuan Liu,Mahesha M. Poojary,Ling Zhu,Andrew R. Williams,Marianne N. Lund
出处
期刊:Journal of Agricultural and Food Chemistry [American Chemical Society]
卷期号:71 (5): 2344-2355 被引量:11
标识
DOI:10.1021/acs.jafc.2c06658
摘要

Caffeic acid (CA) and chlorogenic acid (CGA) are commonly found phenolic acids in plant-derived foods and beverages. Their corresponding adducts with cysteine (Cys) have been detected in coffee-containing beverages. However, despite the well-documented antioxidant and anti-inflammatory activity of CA and CGA, the immunomodulatory activities of the Cys adducts (CA-Cys and CGA-Cys) are unknown. The adducts were therefore synthesized, and their immunomodulatory effects were studied in lipopolysaccharide (LPS)-treated RAW 264.7 cells and compared to the activity of the parent phenolic acids. CA and CGA generally down-regulated the inflammatory responses. However, RNA-sequencing showed that the LPS-induced pathways related to Toll-like receptor signaling, chemokine signaling, and NOD-like receptor signaling, and JAK-STAT/MAPK signaling pathways were upregulated in adduct-treated cells relative to parent phenolic acids, while neurodegenerative disorder-related pathways and metabolic pathways were downregulated. Production of prostaglandin E2 (PGE2), interleukin-6, tumor necrosis factor-α (TNF-α), and reactive oxygen species (ROS) was all inhibited by CA and CGA (P < 0.05). PGE2 and TNF-α were further suppressed in adduct-stimulated cells (P < 0.05), but ROS production was increased. For example, TNF-α produced by 100 μM CGA-stimulated cells and 100 μM CGA-Cys adduct-stimulated cells were 4.46 ± 0.23 and 1.61 ± 0.18 ng/mL, respectively. Thus, the addition of the Cys moiety drastically alters the anti-inflammatory activity of phenolic compounds.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
不爱吃鱼的猫完成签到,获得积分10
1秒前
月亮完成签到 ,获得积分10
1秒前
影像大侠完成签到,获得积分10
3秒前
踢球的孩子完成签到 ,获得积分10
5秒前
美丽的鞋垫完成签到 ,获得积分10
8秒前
nusiew完成签到,获得积分10
8秒前
tonydymt完成签到 ,获得积分10
9秒前
chenying完成签到 ,获得积分0
10秒前
mojomars完成签到,获得积分10
10秒前
TiY完成签到 ,获得积分10
11秒前
12秒前
zoe完成签到 ,获得积分10
14秒前
蓝意完成签到,获得积分0
16秒前
22秒前
雪白发卡完成签到,获得积分10
24秒前
xiaxiao完成签到,获得积分0
27秒前
田野的小家庭完成签到 ,获得积分10
28秒前
一隅完成签到 ,获得积分10
29秒前
刻苦的新烟完成签到 ,获得积分10
30秒前
六沉完成签到 ,获得积分10
31秒前
高挑的若雁完成签到 ,获得积分10
31秒前
siqilinwillbephd完成签到 ,获得积分10
32秒前
celia完成签到 ,获得积分10
36秒前
甚也完成签到 ,获得积分10
36秒前
你帅你有理完成签到,获得积分10
36秒前
阳佟水蓉完成签到,获得积分10
38秒前
一顿吃不饱完成签到,获得积分0
44秒前
99完成签到,获得积分10
46秒前
Sunnpy完成签到 ,获得积分10
47秒前
完美世界应助何博采纳,获得10
48秒前
allzzwell完成签到 ,获得积分10
50秒前
琥1完成签到,获得积分10
51秒前
幸福妙柏完成签到 ,获得积分10
52秒前
执着的忆雪完成签到 ,获得积分10
53秒前
mcl发布了新的文献求助10
54秒前
Hina完成签到,获得积分10
56秒前
Ander完成签到 ,获得积分10
57秒前
niumi190完成签到,获得积分0
1分钟前
孤海未蓝完成签到,获得积分10
1分钟前
Jasper应助科研通管家采纳,获得10
1分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968578
求助须知:如何正确求助?哪些是违规求助? 3513391
关于积分的说明 11167428
捐赠科研通 3248822
什么是DOI,文献DOI怎么找? 1794499
邀请新用户注册赠送积分活动 875116
科研通“疑难数据库(出版商)”最低求助积分说明 804664