检出限
免疫分析
荧光
微流控
残留物(化学)
色谱法
化学
微流控芯片
碳纤维
分析化学(期刊)
材料科学
纳米技术
有机化学
物理
抗体
复合材料
免疫学
复合数
生物
量子力学
作者
Guangming Li,Chen Liu,Xingcai Zhang,Peng Zhai,Xinyi Lai,Wenxiao Jiang
标识
DOI:10.1016/j.bios.2023.115187
摘要
In this study, the N-doped carbon dots were continuously synthesized by a facile microfluidic strategy at 90 °C, and their quantum yields reached 19.2%. The characteristics of the obtained carbon dots could be real-time monitored in order to synthesize carbon dots with specific properties. By incorporating the carbon dots into a well-established enzymatic cascade amplification system, an inner filter effect-based fluorescence immunoassay was set up for ultrasensitive detection of cefquinome residues in milk samples. The developed fluorescence immunoassay provided a low detection limit of 0.78 ng/mL, which satisfied the maximum residue limit set by authorities. The fluorescence immunoassay had an 50% inhibition concentration of 0.19 ng/mL against cefquinome and showed a good linear relationship from 0.013 ng/mL to 1.52 ng/mL. While, the average recovery values ranged from 77.8% to 107.8% in spiked milk samples, with relative standard deviations ranging from 6.8% to 10.9%. Compared with conventional methods, the microfluidic chip was more flexible on carbon dots synthesis and the developed fluorescence immunoassay was more sensitive and eco-friendlier for ultra-trace cefquinome residue analysis.
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