萜烯
萜类
底盘
链霉菌
基因
放线菌门
生物化学
生物
细菌
工程类
遗传学
结构工程
16S核糖体RNA
作者
Yi Ling Hu,Qi Zhang,Shuang He Liu,Jia Sun,Fang Zhou Yin,Zi Ru Wang,Jing Shi,Rui Jiao,Hui Ming Ge
出处
期刊:Chemical Science
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:14 (13): 3661-3667
被引量:9
摘要
Terpenoids comprise the most chemically and structurally diverse family of natural products. In contrast to the huge numbers of terpenoids discovered from plants and fungi, only a relatively small number of terpenoids were reported from bacteria. Recent genomic data in bacteria suggest that a large number of biosynthetic gene clusters encoding terpenoids remain uncharacterized. In order to enable the functional characterization of terpene synthase and relevant tailoring enzymes, we selected and optimized an expression system based on a Streptomyces chassis. Through genome mining, 16 distinct bacterial terpene biosynthetic gene clusters were selected and 13 of them were successfully expressed in the Streptomyces chassis, leading to characterization of 11 terpene skeletons including three new ones, representing an ∼80% success rate. In addition, after functional expression of tailoring genes, 18 novel distinct terpenoids were isolated and characterized. This work demonstrates the advantages of a Streptomyces chassis which not only enabled the successful production of bacterial terpene synthases, but also enabled functional expression of tailoring genes, especially P450, for terpenoid modification.
科研通智能强力驱动
Strongly Powered by AbleSci AI