Tetrastigma hemsleyanum Diels et Gilg, a plant of the Vitaceae family, is often used as a medicine. Modern pharmacological studies have found that its root tubers have a particular anticancer effect. Polydatin, piceatannol, resveratrol, and kaempferol could be detected stably in this herbal plant, which are vital indicators for controlling and evaluating its quality. At present, its planting mode is chaotic and complicated. In order to explore the optimal cultivation mode with excellent quality and yield, the agronomic indicators, the indicators of conventional medical materials and the content of active ingredients were determined in this study. Then, their discrepancy under different planting bases and various planting modes was analyzed. The T. hemsleyanum cultivated in the greenhouse had the thickest stems and the longest leaves, as well as the largest aerial and underground biomass. Compared with understory planting, the root tubers have higher total flavonoid content in the greenhouse planting mode. In addition, the index component content of the stereoscopic planting was higher than that of the crawl planting. In summary, greenhouse planting combined with stereoscopic planting mode was conducive to improving the yield and quality of T. hemsleyanum simultaneously. These results provided a more comprehensive reference basis for the artificial planting of T. hemsleyanum. Simultaneously, it provided a theoretical basis for strengthening the sustainable development and utilization of medicinal materials from T. hemsleyanum and protection of resources.