蛋白酶
烟草蚀刻病毒
劈理(地质)
融合蛋白
融合
生物化学
化学
底物特异性
生物
计算生物学
酶
细胞生物学
重组DNA
病毒
遗传学
马铃薯Y病毒
植物病毒
基因
古生物学
语言学
哲学
断裂(地质)
作者
Lioba Inken Winckler,Nico Dißmeyer
出处
期刊:Methods in Enzymology
日期:2023-01-01
卷期号:: 125-141
被引量:1
标识
DOI:10.1016/bs.mie.2023.02.015
摘要
The tobacco etch virus (TEV) protease is widely used in in vitro and in vivo approaches for the removal of affinity tags from fusion proteins or the generation of proteins with a desired N-terminal amino acid. Processing of fusion proteins by the TEV protease can either be achieved by encoding the TEV protease and its recognition site on one construct (self-cleavage) or on two different constructs (co-expression). Here, we compare the efficiency of the self-splitting approach to the co-expression approach.
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