A SNP mutation in the CsCLAVATA1 leads to pleiotropic variation in plant architecture and fruit morphogenesis in cucumber (Cucumis sativus L.)

Plant architectures is predominantly determined by branching pattern, internode elongation, phyllotaxis, shoot determinacy and reproductive organs. Domestication or improvement of this critical agronomic trait played an important role in the breakthrough of crop yield. Here, we identified a mutant with fasciated plant architecture, named fas, from an ethyl methanesulfonate (EMS) induced mutant population in cucumber. The mutant exhibited abnormal phyllotaxy, flattened main stem, increased number of floral organs, and significantly shorter and thicker fruits. However, the molecular mechanism conferring this pleiotropic effect remains unknown. Using a map-based cloning strategy, we isolated the gene CsaV3_3G045960, encoding a leucine-rich repeat receptor-like kinase, a putative direct homolog of the Arabidopsis CLAVATA1 protein referred to as CsCLV1. Endogenous hormone assays showed that IAA and GA3 levels in fas stems and ovaries were significantly reduced. Conformably, RNA-seq analysis showed that CsCLV1 regulates cucumber stem and ovary development by coordinating hormones and transcription factors. Our results contribute to the understanding of the function of CsCLV1 throughout the growth cycle, provide new evidence that the CLV signaling system is functionally conserved in Cucurbitaceae.