Antioxidant Silk Fibroin Composite Hydrogel for Rapid Healing of Diabetic Wound

Abstract Wound healing is a complex process requiring multiple biological pathways and chemical responses to be activated and synchronized to recover tissue integrity. In normal physiological circumstances, the epidermal barrier restoration process through new tissue formation is highly efficient. However, increased production of reactive oxygen species (ROS), attack of pathogenic microorganisms, and high glucose level delay the normal healing process in diabetic patients. The successful treatment of diabetic wounds requires efficient strategies to control oxidative stress, promoting angiogenesis, re‐epithelialization, and collagen deposition. In this study, a composite hydrogel for rapid wound healing in diabetic condition is developed by the amalgamation of hypolipidemic property of silk fibroin (SF), antioxidant property of melanin, and therapeutic effect of berberine. Studies have revealed that cross‐linked mesoporous morphology of hydrogel matrix facilitates slow release of berberine to impart long‐term therapeutic effects at wound site. The composite hydrogel formulation is biocompatible, stimulates effective migration of fibroblast cells, and control oxidative stress under in vitro conditions. The hydrogel served as scaffold for tissue re‐epithelialization and promotes wound repair in diabetic type I Wistar rat model. This study demonstrates the ability of berberine‐ loaded SF‐melanin composite hydrogel as a potential dressing formulation for wound healing in diabetic conditions.