Novel miRNA markers and their mechanism of esophageal squamous cell carcinoma (ESCC) based on TCGA

食管鳞状细胞癌 小RNA 癌症研究 机制(生物学) 基底细胞 肿瘤科 医学 内科学 生物 基因 遗传学 哲学 认识论
作者
Ping Yuan,Xiaoyan Gao,Mingjun Xu,Liangyu Qiu,Zi Xiong,Jun Shen,Huanhuan Xing,Ruofan Yang,Liang Zhao,Xi Liu,Jiaowei Gu,Wenting Liu
出处
期刊:Scientific Reports [Nature Portfolio]
卷期号:14 (1)
标识
DOI:10.1038/s41598-024-76321-0
摘要

MicroRNAs(miRNAs) are promising biomarkers for early esophageal squamous cell carcinoma (ESCC) detection and prognostic prediction. This study aimed to explore the potential biomarkers and molecular pathogenesis in the early diagnosis of ESCC. Firstly, 48 differentially expressed miRNAs (DEMs) and 1319 differentially expressed genes (DEGs) were identified between 94 ESCC tissues and 13 normal esophageal tissues in TCGA. From miRNA-mRNA regulatory network, there are 6558 target genes of the 48 DEMs, where 400 target genes are also among 1319 DEGs. Then, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment indicate that the 400 DEGs significantly enriched in cell cycle, proteoglycans in cancer, p53 signaling pathway, protein digestion and absorption, transcriptional dysregulation in cancer, and oocyte meiosis. And there are 66 DEGs among these six biological pathways, which we called GO-DEGs. From miRNA-mRNA regulatory network, 32 DEMs regulated the 66 GO-DEGs, where 22 DEMs were verified by different types of experiments in ESCC tissues, cells, or serum from the literature. For the other novel 10 DEMs, single-factor Cox regression analysis show that only hsa-miR-34b-3p showed no significant correlation with the overall survival of ESCC patients. Finally, we obtained the novel 9 ESCC-related DEMs, where three are down-regulated, and six are up-regulated. We analyzed the expression trends of target genes for five miRNAs and identified three significantly different miRNAs (hsa-miR-205-3p, hsa-miR-452-3p, and hsa-miR-6499-3p) confirmed by qPCR. Moreover, the stage-specific miRNAs were also suggested. These three qPCR validated miRNAs are also specific to the early stages of ESCC: hsa-miR-452-3p is specific to Stage I, II and III; hsa-miR-205-3p is specific in Stage II and III; and hsa-miR-6499-3p is Stage II specific. They might be the potential biomarkers for ESCC stage diagnosis. This study identified three novel miRNA markers potentially related to the diagnosis of ESCC and participated in the occurrence and development of ESCC through cell cycle, proteoglycans in cancer, p53 signaling pathway, protein digestion and absorption, transcriptional dysregulation in cancer, and signaling pathway for oocyte meiosis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
善学以致用应助小周周采纳,获得10
刚刚
GXLong发布了新的文献求助10
1秒前
Hey发布了新的文献求助20
1秒前
1秒前
2秒前
柯米克发布了新的文献求助10
2秒前
lm发布了新的文献求助10
5秒前
CipherSage应助体贴汽车采纳,获得10
7秒前
两味愚发布了新的文献求助10
7秒前
8秒前
10秒前
小马甲应助GXLong采纳,获得10
10秒前
10秒前
CodeCraft应助柯米克采纳,获得10
10秒前
深情安青应助淡淡梦容采纳,获得10
11秒前
苏利文发布了新的文献求助30
12秒前
JayeChen完成签到,获得积分10
12秒前
12秒前
屈绮兰应助张张采纳,获得30
13秒前
ding应助玉小赤采纳,获得10
13秒前
14秒前
愉快的雪珍完成签到,获得积分10
15秒前
sylnd126发布了新的文献求助10
15秒前
15秒前
KK发布了新的文献求助10
16秒前
17秒前
19秒前
体贴汽车发布了新的文献求助10
19秒前
20秒前
能干冰露发布了新的文献求助10
20秒前
21秒前
22秒前
liz_应助mariawang采纳,获得10
22秒前
DT发布了新的文献求助10
22秒前
EED驳回了今后应助
23秒前
Orange应助酷酷的王采纳,获得10
23秒前
斯文败类应助达达尼尔采纳,获得10
23秒前
量子星尘发布了新的文献求助10
23秒前
24秒前
25秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988732
求助须知:如何正确求助?哪些是违规求助? 3531027
关于积分的说明 11252281
捐赠科研通 3269732
什么是DOI,文献DOI怎么找? 1804764
邀请新用户注册赠送积分活动 881869
科研通“疑难数据库(出版商)”最低求助积分说明 809021