Comparative population pangenomes reveal unexpected complexity and fitness effects of structural variants
结构复杂性
进化生物学
生物
人口
计算生物学
生态学
人口学
社会学
作者
Scott V. Edwards,Bohao Fang,Danielle E. Khost,George Kolyfetis,Rebecca Cheek,Devon A. DeRaad,Nancy Chen,John W. Fitzpatrick,John E. McCormack,W. Chris Funk,Cameron K. Ghalambor,Erik Garrison,Andrea Guarracino,Heng Li,Timothy B. Sackton
标识
DOI:10.1101/2025.02.11.637762
摘要
Structural variants (SVs) are widespread in vertebrate genomes, yet their evolutionary dynamics remain poorly understood. Using 45 long-read de novo genome assemblies and pangenome tools, we analyze SVs within three closely related species of North American jays (Aphelocoma, scrub-jays) displaying a 60-fold range in effective population size. We find rapid evolution of genome architecture, including ~100 Mb variation in genome size driven by dynamic satellite landscapes with unexpectedly long (> 10 kb) repeat units and widespread variation in gene content, influencing gene expression. SVs exhibit slightly deleterious dynamics modulated by variant length and population size, with strong evidence of adaptive fixation only in large populations. Our results demonstrate how population size shapes the distribution of SVs and the importance of pangenomes to characterizing genomic diversity.