病菌
寄主(生物学)
生物
病毒学
疟原虫(生命周期)
分辨率(逻辑)
微生物学
计算生物学
寄生虫寄主
遗传学
计算机科学
人工智能
万维网
作者
Franziska Hildebrandt,Miren Urrutia Iturritza,C. Zwicker,Bavo Vanneste,Noémi Van Hul,Elisa Semle,Jaclyn Quin,Tales Vicari Pascini,Sami Saarenpää,Mengxiao He,Emma Andersson,Charlotte L. Scott,Joel Vega-Rodríguez,Joakim Lundeberg,Johan Ankarklev
标识
DOI:10.1038/s41467-024-51418-2
摘要
Abstract Upon infecting its vertebrate host, the malaria parasite initially invades the liver where it undergoes massive replication, whilst remaining clinically silent. The coordination of host responses across the complex liver tissue during malaria infection remains unexplored. Here, we perform spatial transcriptomics in combination with single-nuclei RNA sequencing over multiple time points to delineate host-pathogen interactions across Plasmodium berghei- infected liver tissues. Our data reveals significant changes in spatial gene expression in the malaria-infected tissues. These include changes related to lipid metabolism in the proximity to sites of Plasmodium infection, distinct inflammation programs between lobular zones, and regions with enrichment of different inflammatory cells, which we term ‘inflammatory hotspots’. We also observe significant upregulation of genes involved in inflammation in the control liver tissues of mice injected with mosquito salivary gland components. However, this response is considerably delayed compared to that observed in P. berghei- infected mice. Our study establishes a benchmark for investigating transcriptome changes during host-parasite interactions in tissues, it provides informative insights regarding in vivo study design linked to infection and offers a useful tool for the discovery and validation of de novo intervention strategies aimed at malaria liver stage infection.
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