Review of covalent organic frameworks for enzyme immobilization: Strategies, applications, and prospects

Efficient enzyme immobilization systems offer a promising approach for improving enzyme stability and recyclability, reducing enzyme contamination in products, and expanding the applications of enzymes in the biomedical field. Covalent organic frameworks (COFs) possess high surface areas, ordered channels, optional building blocks, highly tunable porosity, stable mechanical properties, and abundant functional groups, making them ideal candidates for enzyme immobilization. Various COF-enzyme composites have been successfully synthesized, with performances that surpass those of free enzymes in numerous ways. This review aims to provide an overview of current enzyme immobilization strategies using COFs, highlighting the characteristics of each method and recent research applications. The future opportunities and challenges of enzyme immobilization technology using COFs are also discussed.