二硫键
材料科学
自组装
组织工程
纳米技术
二硫化钼
血小板
化学工程
生物医学工程
化学
生物化学
复合材料
免疫学
生物
工程类
作者
Maria C. Gomes,Ana Rita Pinho,Catarina A. Custódio,João F. Mano
标识
DOI:10.1002/adma.202304659
摘要
Abstract There is a demand to design microparticles holding surface topography while presenting inherent bioactive cues for applications in the biomedical and biotechnological fields. Using the pool of proteins present in human‐derived platelet lysates (PLs), the production of protein‐based microparticles via a simple and cost‐effective method is reported, exploring the prone redox behavior of cysteine (Cy‐SH) amino acid residues. The forced formation of new intermolecular disulfide bonds results in the precipitation of the proteins as spherical, pompom‐like microparticles with adjustable sizes (15–50 µm in diameter) and surface topography consisting of grooves and ridges. These PL microparticles exhibit extraordinary cytocompatibility, allowing cell‐guided microaggregates to form, while also working as injectable systems for cell support. Early studies also suggest that the surface topography provided by these PL microparticles can support osteogenic behavior. Consequently, these PL microparticles may find use to create live tissues via bottom‐up procedures or injectable tissue‐defect fillers, particularly for bone regeneration, with the prospect of working under xeno‐free conditions.
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