离体
体内
分泌物
细胞生物学
归巢(生物学)
干细胞
等离子体电池
骨髓
生物
免疫学
生物化学
生态学
生物技术
作者
Yu-Hong Cheng,King L. Hung,Tingting Zhang,Claire M. Stoffers,Andee R. Ott,Emmaline R. Suchland,Nathan D. Camp,Iram Khan,Swati Singh,Ying-Jen Yang,David J. Rawlings,Richard G. James
标识
DOI:10.1038/s41467-022-33787-8
摘要
Abstract Due to their unique longevity and capacity to secrete high levels of protein, plasma B cells have the potential to be used as a cell therapy for protein replacement. Here, we show that ex vivo engineered human plasma cells exhibit single-cell RNA profiles, scanning electron micrograph ultrastructural features, and in vivo homing capacity of long-lived plasma cells. After transferring human plasma cells to immunodeficient mice in the presence of the human cytokines BAFF and IL-6, we observe increases in retention of plasma cells in the bone marrow, with engraftment exceeding a year. The most profound in vivo effects of human IL-6 are observed within 20 days of transfer and could be explained by decreased apoptosis in newly differentiated plasma cells. Collectively, these results show that ex vivo engineered and differentiated human plasma cells have the potential for long-lived in vivo protein secretion, which can be modeled in small animals.
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