脱落酸
转录因子
生物
普通大麦
转录组
发起人
转基因作物
基因
基因表达
转基因
植物
细胞生物学
生物化学
禾本科
作者
Aleksandra Smoczyńska,Andrzej Pacak,Aleksandra Grabowska,Dawid Bielewicz,Marcin Zadworny,Kashmir Singh,Jakub Dolata,Mateusz Bajczyk,Przemysław Nuc,Jacek Kęsy,Magdalena Woźniak,Izabela Ratajczak,Wendy Harwood,Wojciech M. Karłowski,Artur Jarmołowski,Zofia Szweykowska-Kulińska
标识
DOI:10.3389/fpls.2022.950796
摘要
Nitrogen (N) is an important element for plant growth and development. Although several studies have examined plants' response to N deficiency, studies on plants' response to excess N, which is common in fertilizer-based agrosystems, are limited. Therefore, the aim of this study was to examine the response of barley to excess N conditions, specifically the root response. Additionally, genomic mechanism of excess N response in barley was elucidated using transcriptomic technologies. The results of the study showed that barley MADS27 transcription factor was mainly expressed in the roots and its gene contained N-responsive cis-regulatory elements in the promoter region. Additionally, there was a significant decrease in HvMADS27 expression under excess N condition; however, its expression was not significantly affected under low N condition. Phenotypic analysis of the root system of HvMADS27 knockdown and overexpressing barley plants revealed that HvMADS27 regulates barley root architecture under excess N stress. Further analysis of wild-type (WT) and transgenic barley plants (hvmads27 kd and hvmads27 c-Myc OE) revealed that HvMADS27 regulates the expression of HvBG1 β-glucosidase, which in turn regulates abscisic acid (ABA) level in roots. Overall, the findings of this study showed that HvMADS27 expression is downregulated in barley roots under excess N stress, which induces HvBG1 expression, leading to the release of ABA from ABA-glucose conjugate, and consequent shortening of the roots.
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