DNA
合成生物学
聚合酶
分子开关
计算生物学
限制性酶
DNA连接酶
DNA折纸
DNA聚合酶
酶
核酸内切酶
DNA纳米技术
化学
纳米技术
生物
生物物理学
细胞生物学
生物化学
材料科学
分子
有机化学
作者
Hyeonbae Kang,Yuande Yang,Bryan Wei
标识
DOI:10.1038/s41467-024-47742-2
摘要
Abstract Taking inspiration from natural systems, in which molecular switches are ubiquitous in the biochemistry regulatory network, we aim to design and construct synthetic molecular switches driven by DNA-modifying enzymes, such as DNA polymerase and nicking endonuclease. The enzymatic treatments on our synthetic DNA constructs controllably switch ON or OFF the sticky end cohesion and in turn cascade to the structural association or disassociation. Here we showcase the concept in multiple DNA nanostructure systems with robust assembly/disassembly performance. The switch mechanisms are first illustrated in minimalist systems with a few DNA strands. Then the ON/OFF switches are realized in complex DNA lattice and origami systems with designated morphological changes responsive to the specific enzymatic treatments.
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