Identifying the regulatory network of microRNAs and mRNAs to clarify molecular mechanisms in stroke by bioinformatics analysis

小RNA 生物 基因 计算生物学 小桶 基因调控网络 转录因子 信号转导 生物信息学 基因表达 遗传学 转录组
作者
Gengyu Cen,Yumei Xia,Zhijian Liang
出处
期刊:Technology and Health Care [IOS Press]
卷期号:: 1-12
标识
DOI:10.3233/thc-231357
摘要

BACKGROUND: Stroke is one of the leading causes of disability and mortality worldwide. OBJECTIVE: To identify the regulatory network of microRNAs (miRNAs) and mRNAs to clarify molecular mechanisms in stroke. METHODS: Four miRNA datasets and two mRNA datasets of stroke were downloaded from the GEO database. R-Studio was utilized to analyze differentially expressed miRNAs (DEmiRNAs) and mRNAs (DEmRNAs) in the blood of stroke and control patients. FunRich software was utilized to conduct GO and biological pathway analysis on DEmiRNAs, and to search for transcription factors (TFs) of DEmiRNAs. Subsequently, we used miRDB, miRTarBase, and TargetScan to identify DEmiRNAs target genes and intersected with DEmRNAs to find common target genes. The miRNA-mRNA regulatory network of common target genes was constructed by using the Cytoscape. The biological and functional roles of target genes in the regulatory network were predicted using GO and KEGG pathway analyses. RESULTS: 464 DEmiRNAs and 329 DEmRNAs were screened. The top ten TFs (SP1, SP4, EGR1, TCF3, NKX6-1, ZFP161, RREB1, MEF2A, NFIC, POU2F1) were visualized. 16747 target genes of DEmiRNAs were predicted. Target genes were intersected with DEmRNAs, 107 common target genes and 162 DEmiRNAs regulating these common genes were obtained, and then a regulatory network was constructed. Target genes of the regulatory network were primarily enriched in VEGF signaling pathway, lipid and atherosclerosis, T cell receptor signaling pathway. CONCLUSION: This study found that VEGF signaling pathway, lipid and atherosclerosis, T cell receptor signaling pathway are implicated in the biological process of stroke by constructing the regulatory network of miRNAs-mRNAs, which may have guide significance for the pathogenesis and treatment of stroke.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
天才幸运鱼完成签到,获得积分10
刚刚
张豪完成签到,获得积分20
刚刚
刚刚
阿南发布了新的文献求助10
1秒前
晴天完成签到,获得积分10
1秒前
db发布了新的文献求助10
1秒前
1秒前
领导范儿应助等待惜文采纳,获得10
1秒前
2秒前
2秒前
2秒前
3秒前
李李李发布了新的文献求助10
3秒前
Johnlian完成签到 ,获得积分10
3秒前
鲁酷发布了新的文献求助10
3秒前
抹茶麻薯发布了新的文献求助10
3秒前
4秒前
科目三应助王w采纳,获得10
4秒前
爱笑觅柔完成签到,获得积分10
4秒前
Daisy完成签到 ,获得积分10
4秒前
英姑应助史塔西采纳,获得10
4秒前
DrHu完成签到,获得积分10
4秒前
Ava应助lan采纳,获得30
4秒前
hyominhsu完成签到,获得积分10
4秒前
HHY关闭了HHY文献求助
4秒前
张宏宇发布了新的文献求助10
4秒前
宏hong发布了新的文献求助30
5秒前
顾矜应助我爱科研采纳,获得10
5秒前
5秒前
缪缪完成签到,获得积分10
6秒前
科研通AI5应助小白采纳,获得10
6秒前
李博士发布了新的文献求助10
6秒前
7秒前
小侯发布了新的文献求助10
7秒前
7秒前
7秒前
8秒前
8秒前
苏甜发布了新的文献求助10
8秒前
jkdi发布了新的文献求助10
9秒前
高分求助中
Technologies supporting mass customization of apparel: A pilot project 600
Chinesen in Europa – Europäer in China: Journalisten, Spione, Studenten 500
Arthur Ewert: A Life for the Comintern 500
China's Relations With Japan 1945-83: The Role of Liao Chengzhi // Kurt Werner Radtke 500
Two Years in Peking 1965-1966: Book 1: Living and Teaching in Mao's China // Reginald Hunt 500
Introduction to Strong Mixing Conditions Volumes 1-3 500
Understanding Interaction in the Second Language Classroom Context 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3808831
求助须知:如何正确求助?哪些是违规求助? 3353506
关于积分的说明 10365583
捐赠科研通 3069749
什么是DOI,文献DOI怎么找? 1685746
邀请新用户注册赠送积分活动 810704
科研通“疑难数据库(出版商)”最低求助积分说明 766300