明胶
透明质酸
硫酸软骨素
脚手架
化学
生物医学工程
间充质干细胞
极限抗拉强度
材料科学
十二烷基硫酸钠
硫酸庆大霉素
伤口愈合
生物物理学
色谱法
生物化学
庆大霉素
细胞生物学
解剖
抗生素
糖胺聚糖
外科
生物
复合材料
医学
作者
Xiaoliang He,Shuai Wang,Xinran Yu,Xiaohui Zhou
标识
DOI:10.1177/08853282231180714
摘要
The repair and functional reconstruction of large skin defects caused by burn remains an intractable clinical problem. Collagen type I (ColI) was extracted from carp scales and confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis ultraviolet adsorption spectra and automatic amino acid analyzer. Then the scaffolds containing the purified ColI, hyaluronic acid (HA) and chondroitin sulfate (CS) were constructed and examined. The results showed that the scaffold (ColI:CS:HA=9:1:1) had larger pore diameter, porosity, water absorption, degradation rate and tensile strength. gentamycin sulphate (GS) - gelatin microspheres (GMSs) were prepared by emulsion cross-linking method. The drug release study of the ColI-CS-HA-GS/GMSs scaffold with antibacterial property showed a prolonged, continuous, and sustained release of GS. The bone marrow mesenchymal stem cells (BMSCs) were extracted from rat and inoculated into the ColI-HA-CS-GS/GMSs scaffold. The results performed that the scaffold could accelerate proliferation of the BMSCs and wound healing.
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