核小体
连接器DNA
染色质
细胞生物学
螺线管
染色体
瑞士/瑞士法郎
生物
染色质重塑
DNA
染色质结构重塑复合物
嘉雅宠物
生物物理学
遗传学
化学
物理
量子力学
作者
Lifei Li,Kangjing Chen,Youyang Sia,Pengjing Hu,Youpi Ye,Zhucheng Chen
标识
DOI:10.1101/2023.01.02.522444
摘要
Nucleosomes are basic repeating units of chromatin, and form regularly spaced arrays in cells. Chromatin remodelers alter the positions of nucleosomes, and are vital in regulating chromatin organization and gene expression. Here we report the cryoEM structure of chromatin remodeler ISW1a complex bound to the dinucleosome. Each subunit of the complex recognizes a different nucleosome. The motor subunit binds to the mobile nucleosome and recognizes the acidic patch through two arginine residues, and the DNA-binding module interacts with the entry DNA at the nucleosome edge. This nucleosome-binding mode provides the structural basis for linker DNA sensing of the motor. Notably, the Ioc3 subunit recognizes the disk face of the adjacent nucleosome through the H4 tail, the acidic patch and the nucleosomal DNA, which is important for the spacing activity in vitro, and for nucleosome organization and cell fitness in vivo. Together, these findings support the nucleosome spacing activity of ISW1a, and add a new mode of nucleosome remodeling in the context of a chromatin environment.
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