A smart ratiometric fluoresence and colorimetry dual-responsive sensor for morin determination based on the complex between carbon quantum dots and polyethyleneimine

化学 检出限 碳量子点 荧光 莫林 猝灭(荧光) 光化学 核化学 色谱法 光学 医学 物理 病理
作者
Xin-He Duan,Hongwei Li,Yuqing Wu
出处
期刊:Analytica Chimica Acta [Elsevier BV]
卷期号:1243: 340814-340814 被引量:13
标识
DOI:10.1016/j.aca.2023.340814
摘要

As a representative flavonoid, morin exhibits multi-biological activities, but its abuse endangers human health. Developing advanced technology for morin determination is urgently needed. In this study, a dual-responsive approach was reported for morin based on the complexing of carbon quantum dots (CQDs) and polyethyleneimine (PEI). The CQDs were fabricated via an improved hydrothermal method employing tyrosine and malic acid. Binding with PEI induced an 8-fold emission enhancement and a slight red-shift to 445 nm of CQDs because of the complexing of PEI and CQDs. Further morin introduction led to the blue emission (445 nm) quenching of CQDs-PEI and a yellow emission (560 nm) generation, which contributed a ratiometric fluorescence approach for morin determination between 2.0 and 32 μM, with a limit of detection (LOD) of 45 nM. Meanwhile, under sunlight the color of CQDs-PEI became yellow upon morin addition, which developed a colorimetric method for morin determination in a wide range between 2.0 and 100 μM (LOD = 69 nM). The developed dual-responsive method either displayed accurate results for morin in diluted human and bovine serum, being potential for actual sample analysis. Finally, a visual detection based on the smartphone was constructed and applied for the real-time determination of morin.
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