生物
Wnt信号通路
房室管
细胞生物学
解剖
内科学
信号转导
心脏病
医学
作者
Chenxi Ye,Changqing Yang,Heqiang Zhang,Rui Gao,Yingnan Liao,Yali Zhang,Ling‐Jun Jie,Yanhui Zhang,Tong Cheng,Yan Wang,Jie Ren
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2024-03-01
卷期号:31 (3): 398-409.e5
被引量:1
标识
DOI:10.1016/j.stem.2024.01.008
摘要
The creation of a functional 3D bioprinted human heart remains challenging, largely due to the lack of some crucial cardiac cell types, including the atrioventricular canal (AVC) cardiomyocytes, which are essential to slow down the electrical impulse between the atrium and ventricle. By utilizing single-cell RNA sequencing analysis and a 3D bioprinting technology, we discover that stage-specific activation of canonical Wnt signaling creates functional AVC cardiomyocytes derived from human pluripotent stem cells. These cardiomyocytes display morphological characteristics and express molecular markers of AVC cardiomyocytes, including transcription factors TBX2 and MSX2. When bioprinted in prefabricated cardiac tissues, these cardiomyocytes successfully delay the electrical impulse, demonstrating their capability of functioning as the AVC cardiomyocytes in vitro. Thus, these findings not only identify canonical Wnt signaling as a key regulator of the AVC cardiomyocyte differentiation in vitro, but, more importantly, provide a critical cellular source for the biofabrication of a functional human heart.
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