Lactiplantibacillus brownii sp. nov., a novel psychrotolerant species isolated from sauerkraut

A Gram-stain-positive, rod-shaped, facultatively anaerobic and homofermentative strain, named WILCCON 0030^T, was isolated from sauerkraut (fermented cabbage) collected from a local market in the Moscow region of Russia. Comparative analyses based on 16S rRNA gene sequence similarity and whole genome relatedness indicated that strain WILCCON 0030^T was most closely related to the type strains Lactiplantibacillus nangangensis NCIMB 15186^T, Lactiplantibacillus daoliensis LMG 31171^T and Lactiplantibacillus pingfangensis LMG 31176^T. However, the average nucleotide identity and digital DNA-DNA hybridization prediction values with these closest relatives only ranged from 84.6 to 84.9 % and from 24.1 to 24.7 %, respectively, and were below the 95.0 and 70.0% thresholds for species delineation. Substantiated by further physiological and biochemical analyses, strain WILCCON 0030^T represents a novel species within the genus Lactiplantibacillus for which we propose the name Lactiplantibacillus brownii sp. nov. (type strain WILCCON 0030^T=DSM 116485^T=LMG 33211^T).