Comparing three Methods of Extraction of Auricularia Auricula Polysaccharides

Auricularia auricula are rich source of bioactive polysaccharides. Three methods of extraction of polysaccharide from these fungi was studied. These methods included, water-based, ultrasonic-assisted, and enzyme-based extractions. The results showed that the enzyme-based extraction was better than ultrasonic-assisted extraction and water extraction. While abundance of polysaccharide concentration in A. auricula-judae and A. delicata were similar, their yield following enzyme-based extractions were higher than that of A. polytricha. The maximum A. auricula-judae polysaccharide concentration in enzyme-assisted extraction was obtained at a pH of 5.0, a temperature of 50°C and 3% cellulase concentration, and the polysaccharide concentration was 52.28 mg/g. The maximum A. delicata polysaccharide concentration (55.16 mg/g) was extracted at a pH of 5.0, a temperature of 50°C and 4% cellulase concentration.