TFBG-SPR DNA-Biosensor for Renewable Ultra-Trace Detection of Mercury Ions

生物传感器 表面等离子共振 分析化学(期刊) 材料科学 DNA 检出限 Mercury(编程语言) 纳米颗粒 化学 纳米技术 色谱法 计算机科学 生物化学 程序设计语言
作者
Yifan Duan,Fang Wang,Xinpu Zhang,Qiang Liu,Mengdi Lu,Wei Ji,Yang Zhang,Zhenguo Jing,Wei Peng
出处
期刊:Journal of Lightwave Technology [Institute of Electrical and Electronics Engineers]
卷期号:39 (12): 3903-3910 被引量:40
标识
DOI:10.1109/jlt.2020.3035003
摘要

In this article, a tilted fiber Bragg grating (TFBG) plasmonic sensor based on thymine (T) - Hg - thymine (T) (T-Hg-T) base asymmetric pairing and Au nanoparticles (AuNPs), which can be used for highly sensitive and selective ultra-low-concentration Hg 2+ detection, has been demonstrated. As is known, TFBG can provide an effective mean to easily excite the surface plasmon resonance (SPR) phenomenon while the fiber is coated with metal layer. By measuring the evolution of spectra, the SPR properties hence the surface perturbation could be detected accurately. To achieve highly sensitive and selective Hg 2+ detection, the two specially designed single-strand probe DNAs (ssDNAs) with rich T base that can specifically forming stable T-Hg-T asymmetric pairing are served as a binding element for AuNPs labelled-target DNA conjugates. In the present of Hg 2+ , these two ssDNAs that modified respectively on the surface of the sensor and the AuNPs will create a double helix T-Hg-T structure between the two DNA oligonucleotides and result in the surface refractive index perturbation amplified by the AuNPs. To overcome the low reusability of the AuNPs based DNA biosensor, by using iodide ions that can form more stable compounds with mercury ions over the T-Hg 2+ -T structure, a regenerable DNA biosensor that can repeatedly detect mercury ions is developed. The experimental results demonstrate that this sensor shows an excellent ultra-low-concentration and selectivity detection capability for Hg 2+ with the limit of detection (LOD) of 3.073 × 10 -12 M (3.073 pM) as well as a large dynamic range of 8 order of magnitude from 1.0 × 10 -11 M to 1.0 × 10 -3 M simultaneously. Thus, the experimental results in various actual sample from ultrapure/tap water to even clinical human serum, also reveal that it has potential application for Hg 2+ detection in environmental samples and in clinic biological samples.
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