细胞外基质
化学
静电纺丝
材料科学
生物物理学
Ⅰ型胶原
基质金属蛋白酶
氨基葡萄糖
粘附
化学工程
基质(化学分析)
糖胺聚糖
纳米技术
生物医学工程
聚合物
生物化学
有机化学
色谱法
工程类
生物
医学
内分泌学
作者
Guomin Wu,Xiao Ma,Yiding Wang,Lina Fan,Yining Wang,Hongbing Deng
标识
DOI:10.1016/j.apsusc.2020.148335
摘要
In order to highly simulate the chondrocyte extracellular matrix, electrospinning and layer-by-layer (LBL) self-assembly techniques were applied in this study. Polycaprolactone (PCL) with good mechanical property was selected as the substrate, while D-glucosamine sulfate (GAS) and collagen type I (COL) with chondroprotective properties were coated on the surfaces of PCL nanofibers. The morphology, chemical and physical investigations suggested the successful deposition of GAS/COL, improved mechanical properties and significantly ameliorated surfaces hydrophilicity after LBL medication. The release of GAS/COL was proved to be slow and sustainable. Besides, CCK-8 assay and cell morphology observation manifested that LBL structured mats were more suitable for rat articular chondrocytes (rACs) adhesion and proliferation, while quantitative Real-Time polymerase chain reaction analysis and glycosaminoglycans (GAG) production measurement indicated the introduction of GAS/COL upregulated glucuronosyltransferase I (GlcAT-I) gene expression and GAG content in rACs. Additionally, LBL structured mats induced GlcAT-I expression and reduced interleukin‐1β (IL‐1β) and Matrix metalloproteinases 13 (MMP13) expression in IL‐1β-induced rACs. Moreover, subcutaneous model in SCID mice further confirmed that GAS/COL modified PCL mat could facilitate the GAG production and collagen type II expression of rACs.
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