荧光寿命成像显微镜
光化学
双分子荧光互补
荧光团
半胱氨酸
绿色荧光蛋白
作者
Zhang-Yi Li,Xiaoling Cui,Yizhou Yan,Qiao-Ling Che,Miao Jiang,Bao‐Xiang Zhao,Zhao‐Min Lin
标识
DOI:10.1016/j.dyepig.2021.109180
摘要
A novel FRET-based fluorescence probe, NBIS, constructed by benzoindole-based hemicyanine as an acceptor and naphthalimide derivatives as a donor was developed for the detecting of sulfur dioxide (SO2) derivatives. Probe NBIS exhibited high energy transfer efficiency (89%) and showed high sensitivity (LOD: 16.2 nM) toward HSO3−/SO32−. Probe NBIS could target the endoplasmic reticulum (ER) and was applied to image exogenous and endogenous HSO3−/SO32− in living cells.
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