Podometrics in Japanese Living Donor Kidneys: Associations with Nephron Number, Age, and Hypertension

肾单位 足细胞 体视学 肾小球硬化 肾脏疾病 局灶节段性肾小球硬化 医学 内科学 泌尿科 内分泌学 人口 病理 肾小球肾炎 蛋白尿 环境卫生
作者
Kotaro Haruhara,Takaya Sasaki,Natasha de Zoysa,Yusuke Okabayashi,Go Kanzaki,Izumi Yamamoto,Ian S. Harper,Victor G. Puelles,Akira Shimizu,Luise A. Cullen‐McEwen,Nobuo Tsuboi,Takashi Yokoo,John F. Bertram
出处
期刊:Journal of The American Society of Nephrology 卷期号:32 (5): 1187-1199 被引量:15
标识
DOI:10.1681/asn.2020101486
摘要

Significance Statement Podocyte depletion and low nephron number are associated with glomerulosclerosis and CKD. However, the relationship between podometrics and nephron number has not previously been reported. The authors estimated podometric parameters and nephron number in 30 Japanese kidney donors. Their podocyte density and number per glomerulus were similar to values reported for other racial groups, whereas they had fewer nonsclerotic nephrons compared with other races. Total podocyte number per kidney declined at a rate of 5.63 million podocytes per year, with 80% of podocyte loss resulting from glomerulosclerosis-associated glomerular loss, and the remainder occurring in healthy glomeruli. Hypertension was associated with lower podocyte density and larger podocyte volume, independent of age. These approaches could be of value in evaluating the kidney in health and disease. Background Podocyte depletion, low nephron number, aging, and hypertension are associated with glomerulosclerosis and CKD. However, the relationship between podometrics and nephron number has not previously been examined. Methods To investigate podometrics and nephron number in healthy Japanese individuals, a population characterized by a relatively low nephron number, we immunostained single paraffin sections from 30 Japanese living-kidney donors (median age, 57 years) with podocyte-specific markers and analyzed images obtained with confocal microscopy. We used model-based stereology to estimate podometrics, and a combined enhanced–computed tomography/biopsy-specimen stereology method to estimate nephron number. Results The median number of nonsclerotic nephrons per kidney was 659,000 (interquartile range [IQR], 564,000–825,000). The median podocyte number and podocyte density were 518 (IQR, 428–601) per tuft and 219 (IQR, 180–253) per 10 6 μ m 3 , respectively; these values are similar to those previously reported for other races. Total podocyte number per kidney (obtained by multiplying the individual number of nonsclerotic glomeruli by podocyte number per glomerulus) was 376 million (IQR, 259–449 million) and ranged 7.4-fold between donors. On average, these healthy kidneys lost 5.63 million podocytes per kidney per year, with most of this loss associated with glomerular loss resulting from global glomerulosclerosis, rather than podocyte loss from healthy glomeruli. Hypertension was associated with lower podocyte density and larger podocyte volume, independent of age. Conclusions Estimation of the number of nephrons, podocytes, and other podometric parameters in individual kidneys provides new insights into the relationships between these parameters, age, and hypertension in the kidney. This approach might be of considerable value in evaluating the kidney in health and disease.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
辣辣完成签到,获得积分10
刚刚
桐桐应助白华苍松采纳,获得10
刚刚
华仔应助啊嚯采纳,获得10
刚刚
yasan完成签到,获得积分10
刚刚
1秒前
Fsy完成签到,获得积分10
1秒前
万能图书馆应助China采纳,获得10
1秒前
杨欢完成签到,获得积分10
1秒前
Stanley发布了新的文献求助10
1秒前
哭泣爆米花完成签到,获得积分10
2秒前
2秒前
2秒前
2秒前
钰宁发布了新的文献求助10
2秒前
灵巧荆发布了新的文献求助10
2秒前
慕青应助juan采纳,获得10
3秒前
3秒前
白小白发布了新的文献求助10
3秒前
丘比特应助阳光莲小蓬采纳,获得10
3秒前
司徒迎曼发布了新的文献求助10
3秒前
3秒前
4秒前
liuliu发布了新的文献求助10
4秒前
4秒前
523发布了新的文献求助10
4秒前
popcorn完成签到,获得积分10
5秒前
C2完成签到,获得积分10
5秒前
Agernon应助小小技术工采纳,获得10
6秒前
Rsoup发布了新的文献求助10
6秒前
九川发布了新的文献求助30
6秒前
漂亮的初蓝完成签到,获得积分10
6秒前
丰知然应助紫菜采纳,获得10
6秒前
科目三应助顺顺采纳,获得20
6秒前
桐桐应助自信富采纳,获得10
7秒前
健忘捕发布了新的文献求助10
7秒前
8秒前
叫滚滚发布了新的文献求助10
8秒前
请叫我风吹麦浪应助rain采纳,获得30
9秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Social media impact on athlete mental health: #RealityCheck 1020
Ensartinib (Ensacove) for Non-Small Cell Lung Cancer 1000
Unseen Mendieta: The Unpublished Works of Ana Mendieta 1000
Bacterial collagenases and their clinical applications 800
El viaje de una vida: Memorias de María Lecea 800
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3527723
求助须知:如何正确求助?哪些是违规求助? 3107826
关于积分的说明 9286663
捐赠科研通 2805577
什么是DOI,文献DOI怎么找? 1539998
邀请新用户注册赠送积分活动 716878
科研通“疑难数据库(出版商)”最低求助积分说明 709762