Optimization of the degradation conditions of swine wastewater and microbial community structure analysis in immobilized microbial technology

Abstract In this paper, the treatment characteristics of immobilized microbial technology on swine wastewater under different operating conditions were investigated, so as to optimize the operating conditions of the system. At the same time, the microorganism community structure in the optimal condition was analyzed by high throughput sequencing technology. As a results, the optimal conditions for the degradation of TN in swine wastewater by immobilized microorganism were the filling rate of 25%, HRT of 27.5h and C/N of 5.48. In this case, the actual value of the TN removal rate was 74.81%. With the operation of the system, the abundance and diversity of the microbial community in the system are decreased, while the diversity and abundance of the microbial community in the denitrification tank are generally higher than that in the nitrification tank. Proteobacteria and Bacteroidetes are the dominant bacterial phylum, Hydrogenophaga are the dominant bacterial genus. The function of microbial community in the system is mainly "Metabolism".