曲古抑菌素A
细胞生物学
间充质干细胞
牙周炎
炎症
化学
生物
免疫学
癌症研究
医学
牙科
组蛋白脱乙酰基酶
生物化学
组蛋白
DNA
作者
Qiong Li,Fan Liu,Rui Dang,Chunyue Feng,Rui Xiao,Yingqi Hua,Wei Wang,Zhi Jia,Dayong Liu
摘要
Abstract We wished to evaluate whether epigenetic modifiers have a beneficial effect on treating experimental periodontitis and mechanisms for regulating the cell fate of mesenchymal stem cells (MSCs) in inflammatory microenvironments. We isolated MSCs from healthy and inflamed gingival tissues to investigate whether trichostatin A (TSA) could improve osteogenic differentiation and resolve inflammation in vitro. The tissue regenerative potentials were evaluated when treated with a temperature‐dependent, chitosan‐scaffold‐encapsulated TSA, in a rat model of periodontitis. After induction with the conditioned medium, TSA treatment increased the osteogenic differentiation potential of inflamed MSCs and healthy MSCs. In addition, interleukin‐6 and interleukin‐8 levels in supernatants were significantly decreased after TSA treatment. Moreover, TSA promoted osteogenic differentiation by inhibiting nuclear factor‐κB (p65) DNA binding in MSCs. In rats with experimental periodontitis, 7 weeks after local injections of chitosan‐scaffold‐encapsulated TSA, histology and microcomputed tomography showed a significant increase in alveolar bone volume and less inflammatory infiltration compared with vehicle‐treated rats. The concentrations of interferon‐γ and interleukin‐6 were significantly decreased in the gingival crevicular fluid after TSA treatment. This study demonstrated that TSA had anti‐inflammatory properties and could promote periodontal tissue repair, which indicated that epigenetic modifiers hold promise as a potential therapeutic option for periodontal tissue repair.
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