生物炼制
水解
多糖
化学
生物量(生态学)
木质纤维素生物量
发酵
木质素磺酸盐
生物燃料
生化工程
制浆造纸工业
生物化学
生物技术
工程类
生物
木质素
有机化学
生态学
作者
Myounghoon Moon,Joon-Pyo Lee,Gwon Woo Park,Jin‐Suk Lee,Hyun June Park,Kyoungseon Min
标识
DOI:10.1016/j.biortech.2022.127501
摘要
Given that traditional biorefineries have been based on microbial fermentation to produce useful fuels, materials, and chemicals as metabolites, saccharification is an important step to obtain fermentable sugars from biomass. It is well-known that glycosidic hydrolases (GHs) are responsible for the saccharification of recalcitrant polysaccharides through hydrolysis, but the discovery of lytic polysaccharide monooxygenase (LPMO), which is a kind of oxidative enzyme involved in cleaving polysaccharides and boosting GH performance, has profoundly changed the understanding of enzyme-based saccharification. This review briefly introduces the classification, structural information, and catalytic mechanism of LPMOs. In addition to recombinant expression strategies, synergistic effects with GH are comprehensively discussed. Challenges and perspectives for LPMO-based saccharification on a large scale are also briefly mentioned. Ultimately, this review can provide insights for constructing an economically viable lignocellulose-based biorefinery system and a closed-carbon loop to cope with climate change.
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