Effect of different forms and components of EPS on sludge aggregation during granulation process of aerobic granular sludge

The characteristics of three forms of extracellular polymeric substances (EPS) and their component proteins (PN) and polysaccharides (PS) during the granulation process of aerobic granular sludge (AGS) and their effects on the aggregation performance of AGS were studied. Meanwhile, Pearson correlation analysis was conducted to explore the correlations between different forms of EPS and their components and sludge properties in the granulation process. The results showed that the AGS reactor could be started within 40 days by using the strategy of decreasing sedimentation time, and the sludge granulation degree was 90.22%. It was found that the content of tightly bound EPS (TB-EPS) accounted for 72.81-93.11% of the total EPS. Compared with dissolved EPS (S-EPS) and loosely bound EPS (LB-EPS), TB-EPS had a great impact on the zeta potential and hydrophobicity of sludge surface. During the granulation process, the content and relative hydrophobicity of PN in TB-EPS increased from 30.38 ± 0.12 mg/g VSS to 83.99 ± 2.05 mg/g VSS and 37.63%-71.25%, respectively. Through the flocculation experiment, it showed that the contribution rate of TB-EPS to the flocculation capacity of sludge cells increased from 13.30% to 35.37% and that of PN to flocculation capacity was 76.93%, which was 6.8 times that of PS. It indicated that TB-EPS played the most critical role in the formation of AGS and the PN in EPS was the major contributor to promoting the formation of AGS and enhance its aggregation performance. This research could further understand the role of various forms of EPS and its components in the process of sludge granulation, reveal the sludge granulation mechanism, and provide theoretical support for the rapid start-up of AGS reactor.