Raman spectral analysis of nasopharyngeal carcinoma cell line CNE2 after microwave radiation

This study aimed to study the effects of microwave radiation on the nasopharyngeal carcinoma cell line CNE2 by Raman spectroscopy. The cells were separated into a control group and radiated groups with radiation times of 2, 5, 10, and 25 min, respectively. Both principal components analysis and support vector machine were employed for statistical analysis of Raman spectra. The results show that the relative content of C-H deformation and amide I begin to change when the radiation time is over 10 min, and principal components analysis further confirms there are significant differences after 10 min of radiation. Moreover, support vector machine is simultaneously used to classify radiated samples from control samples. The classification accuracy is low until the radiation time reaches over 10 min. In conclusion, this study reveals the Raman spectral characteristics of CNE2 under different microwave radiation exposure timesand demonstrates Raman spectroscopy can be a potential method to explore cellular characterization after radiation. The final results may help in elucidating the mechanism by which microwave radiation interacts with tumor cells.