Antibodies to SPARC inhibit albumin binding to SPARC, gp60, and microvascular endothelium

跨细胞 白蛋白 牛血清白蛋白 化学 生物化学 内皮 血清白蛋白 抗体 血浆蛋白结合 分子生物学 生物 免疫学 内吞作用 受体 内分泌学
作者
Jan E. Schnitzer,Philmo Oh
出处
期刊:American Journal of Physiology-heart and Circulatory Physiology [American Physiological Society]
卷期号:263 (6): H1872-H1879 被引量:99
标识
DOI:10.1152/ajpheart.1992.263.6.h1872
摘要

Albumin, through its binding to the endothelial glycocalyx, functions as a major determinant of capillary permeability and as a carrier for various small molecules in its transcytosis across continuous endothelium via plasma-lemmal vesicles. Several albumin-binding proteins (ABP) have been identified: three membrane-associated ABP, which we call gp60, gp30, and gp18, and one secreted protein, acidic and rich in cysteine (SPARC). In this study, we used antiserum raised against bovine SPARC (BON) to investigate the possible interrelationships among ABP to better understand their role in binding and transcytosis. BON not only interacted with SPARC secreted by cultured endothelium but also recognized gp60 in lysates of cultured rat, human, and bovine endothelial cells. Purified SPARC inhibited BON interaction with gp60. BON immunoglobulin (Ig)G specifically inhibited albumin binding to both SPARC and gp60 extracts. This effect was eliminated by preabsorption of BON to immobilized SPARC. BON also significantly inhibited albumin binding to cultured microvascular endothelial cells via its interaction with gp60. Anti-SPARC peptide sera were also tested, and one serum raised against a peptide encompassing an NH2-terminal region of SPARC recognized both SPARC and gp60 but did not inhibit albumin binding; gp30 and gp18 were not recognized by any of these anti-SPARC antibodies. These results suggest that SPARC and gp60 are functionally and immunologically related ABP that may share a common albumin-binding domain. gp60 appears to be the major mediator of albumin binding to microvascular endothelium.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
llp完成签到,获得积分10
1秒前
1秒前
2秒前
3秒前
手心手背发布了新的文献求助10
4秒前
4秒前
4秒前
聪聪发布了新的文献求助10
5秒前
养恩应助执笔采纳,获得10
5秒前
6秒前
向秋发布了新的文献求助10
6秒前
飘逸涛发布了新的文献求助10
7秒前
7秒前
7秒前
guojingjing发布了新的文献求助10
8秒前
高分子发布了新的文献求助10
8秒前
9秒前
10秒前
11秒前
11秒前
12秒前
FashionBoy应助ZhangWen采纳,获得10
12秒前
13秒前
14秒前
阿斯顿风格完成签到,获得积分10
14秒前
14秒前
奕奕发布了新的文献求助10
14秒前
14秒前
16秒前
西安小小朱完成签到,获得积分10
16秒前
iiiau发布了新的文献求助10
16秒前
科研通AI5应助张永明采纳,获得10
18秒前
animenz完成签到,获得积分10
19秒前
所所应助多肉葡萄采纳,获得10
19秒前
Carrido发布了新的文献求助10
19秒前
悦耳的又蓝完成签到,获得积分20
19秒前
刘总发布了新的文献求助10
20秒前
渡渡鸟完成签到,获得积分20
20秒前
豆丁发布了新的文献求助10
20秒前
Yoh1220发布了新的文献求助10
21秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Structural Load Modelling and Combination for Performance and Safety Evaluation 1000
Conference Record, IAS Annual Meeting 1977 820
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
Typology of Conditional Constructions 500
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3570690
求助须知:如何正确求助?哪些是违规求助? 3141392
关于积分的说明 9442819
捐赠科研通 2842740
什么是DOI,文献DOI怎么找? 1562418
邀请新用户注册赠送积分活动 731079
科研通“疑难数据库(出版商)”最低求助积分说明 718290