A dual-signal amplification strategy for kanamycin based on ordered mesoporous carbon-chitosan/gold nanoparticles-streptavidin and ferrocene labelled DNA

An ultrasensitive electrochemical aptasensor for kanamycin (KAN) detection was constructed with a dual-signal amplification strategy. The aptasensor achieved greatly amplified sensitivity due to the excellent electrical conductivity of the ordered mesoporous carbon-chitosan (OMC-CS)/gold nanoparticles-streptavidin (AuNPs-SA) and DNA2 labelled with ferrocene (Fc-DNA2). The AuNPs-SA was used to immobilize the DNA strand (biotin labelled) with the biotin-streptavidin system. The DNA2 strand containing the KAN aptamer was labelled with ferrocene to increase the current signal on the electrode surface when bound to KAN. Some factors that affect the performance of the aptasensor were optimized, and the proposed aptasensor provided a wide linear range from 1 × 10−10 M to 4 × 10−6 M, with a detection limit as low as 35.69 pM for KAN under the optimized conditions. This aptasensor had satisfactory electrochemical performance with good stability, sensitivity and reproducibility. Additionally, it also displayed a good specificity for KAN without interference from competitive analogues. Furthermore, the constructed aptasensor was successfully used to detect KAN in a real milk sample. The proposed method for KAN detection has great potential for the detection of other antibiotics.