化学
检出限
香豆素
荧光
选择性
显色的
同型半胱氨酸
半胱氨酸
线性范围
光化学
灵敏度(控制系统)
谷胱甘肽
分析化学(期刊)
色谱法
生物化学
光学
有机化学
催化作用
物理
工程类
酶
电子工程
作者
Kun‐Peng Wang,Shengnan Xu,Lei Yang,Wenjun Zheng,Qi Zhang,Shaojin Chen,Hai‐Yu Hu,Zhi‐Qiang Hu
出处
期刊:Talanta
[Elsevier]
日期:2018-12-22
卷期号:196: 243-248
被引量:26
标识
DOI:10.1016/j.talanta.2018.12.060
摘要
In this study, a new coumarin-based fluorescent and chromogenic dual channel probe (DC) was used for the selective detection of homocysteine (Hcy) over other amino acids, especially for cysteine (Cys) and glutathione (GSH). When Hcy is present in the solution, the remarkable fluorescence enhancement and obvious blue shift in UV–vis spectra can be observed. In addition, the color change from purple to yellow can be observed clearly by unaided eyes. This probe DC has fast response time, excellent sensitivity and selectivity to Hcy. A linear relationship exists between the ratio of emissions at 486 and 625 nm, and Hcy can be detected in a wide concentration range (0–200 μM). The signal-to-background ratio of fluorescence at 486 nm can reach 8.4, and the detection limit is calculated to be 3.5 µM. The response mechanism is proved to be the Michael addition reaction by Hcy. Preliminary results on cell imaging enable the practical application of Hcy tracing in living cells.
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