Using RDNA sequences to define dinoflagellate species

生物 系统发育树 甲藻 系统发育学 进化生物学 基因 生态学 遗传学
作者
Brittany M. Ott,R. Wayne Litaker,William C. Holland,Charles F. Delwiche
出处
期刊:PLOS ONE [Public Library of Science]
卷期号:17 (2): e0264143-e0264143 被引量:5
标识
DOI:10.1371/journal.pone.0264143
摘要

Dinoflagellate species are traditionally defined using morphological characters, but molecular evidence accumulated over the past several decades indicates many morphologically-based descriptions are inaccurate. This recognition led to an increasing reliance on DNA sequence data, particularly rDNA gene segments, in defining species. The validity of this approach assumes the divergence in rDNA or other selected genes parallels speciation events. Another concern is whether single gene rDNA phylogenies by themselves are adequate for delineating species or if multigene phylogenies are required instead. Currently, few studies have directly assessed the relative utility of multigene versus rDNA-based phylogenies for distinguishing species. To address this, the current study examined D1-D3 and ITS/5.8S rDNA gene regions, a multi-gene phylogeny, and morphological characters in Gambierdiscus and other related dinoflagellate genera to determine if they produce congruent phylogenies and identify the same species. Data for the analyses were obtained from previous sequencing efforts and publicly available dinoflagellate transcriptomic libraries as well from the additional nine well-characterized Gambierdiscus species transcriptomic libraries generated in this study. The D1-D3 and ITS/5.8S phylogenies successfully identified the described Gambierdiscus and Alexandrium species. Additionally, the data showed that the D1-D3 and multigene phylogenies were equally capable of identifying the same species. The multigene phylogenies, however, showed different relationships among species and are likely to prove more accurate at determining phylogenetic relationships above the species level. These data indicated that D1-D3 and ITS/5.8S rDNA region phylogenies are generally successful for identifying species of Gambierdiscus, and likely those of other dinoflagellates. To assess how broadly general this finding is likely to be, rDNA molecular phylogenies from over 473 manuscripts representing 232 genera and 863 described species of dinoflagellates were reviewed. Results showed the D1-D3 rDNA and ITS phylogenies in combination are capable of identifying 97% of dinoflagellate species including all the species belonging to the genera Alexandrium, Ostreopsis and Gambierdiscus, although it should be noted that multi-gene phylogenies are preferred for inferring relationships among these species. A protocol is presented for determining when D1-D3, confirmed by ITS/5.8S rDNA sequence data, would take precedence over morphological features when describing new dinoflagellate species. This protocol addresses situations such as: a) when a new species is both morphologically and molecularly distinct from other known species; b) when a new species and closely related species are morphologically indistinguishable, but genetically distinct; and c) how to handle potentially cryptic species and cases where morphotypes are clearly distinct but have the same rDNA sequence. The protocol also addresses other molecular, morphological, and genetic approaches required to resolve species boundaries in the small minority of species where the D1-D3/ITS region phylogenies fail.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
酸奶花生完成签到 ,获得积分10
刚刚
CT发布了新的文献求助10
1秒前
NexusExplorer应助11111采纳,获得10
2秒前
123完成签到,获得积分10
2秒前
6秒前
酷波er应助冷艳的姿采纳,获得10
7秒前
7秒前
小蘑菇应助缥缈冷安采纳,获得10
7秒前
ccygpp199发布了新的文献求助10
7秒前
NexusExplorer应助佳里采纳,获得10
8秒前
淡淡的向雁完成签到,获得积分10
8秒前
9秒前
9秒前
Jasper应助LJJ采纳,获得10
11秒前
小啦啦3082发布了新的文献求助10
11秒前
11秒前
12秒前
甜甜的悲完成签到,获得积分20
12秒前
今后应助wbh采纳,获得10
12秒前
13秒前
妖妖灵完成签到,获得积分20
13秒前
加油干发布了新的文献求助10
13秒前
彭于晏应助xuan采纳,获得10
13秒前
甜甜的悲发布了新的文献求助10
14秒前
gwenjing发布了新的文献求助10
15秒前
wsj发布了新的文献求助10
17秒前
17秒前
量子星尘发布了新的文献求助10
17秒前
张金蝶发布了新的文献求助10
17秒前
jinzhen发布了新的文献求助10
17秒前
文献发布了新的文献求助30
18秒前
太牛的GGB完成签到,获得积分20
18秒前
19秒前
穿裤子的云完成签到,获得积分10
20秒前
20秒前
今后应助科研通管家采纳,获得10
22秒前
SciGPT应助科研通管家采纳,获得10
22秒前
SYLH应助科研通管家采纳,获得10
22秒前
科研通AI5应助科研通管家采纳,获得10
22秒前
余烬22完成签到,获得积分10
22秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989334
求助须知:如何正确求助?哪些是违规求助? 3531428
关于积分的说明 11253936
捐赠科研通 3270119
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809173