Global metagenomics analyses demonstrate metformin‐induced changes in the gut mycobiome in subjects with type 2 diabetes

二甲双胍 肠道菌群 失调 基因组 生物 2型糖尿病 微生物群 疾病 糖尿病 免疫学 微生物学 医学 生物信息学 内科学 内分泌学 遗传学 基因
作者
Emily Van Syoc,Connie J. Rogers,Erika Ganda
出处
期刊:The FASEB Journal [Wiley]
卷期号:36 (S1)
标识
DOI:10.1096/fasebj.2022.36.s1.r4993
摘要

The fungal component of the gut microbiota (mycobiome) has been less well studied than bacterial populations; however, emerging evidence suggests that the mycobiome might play an important role in metabolic disease. Intestinal fungi play an important role in modulating immune responses at mucosal and systemic sites, but the extent of host-bacterial-fungi interactions in disease pathogenesis remains unknown. Metabolic diseases such as obesity and type 2 diabetes are accompanied by an altered gut microbiome, known as dysbiosis, and recent work suggests that the gut mycobiome may also be perturbed in metabolic diseases. Correcting gut dysbiosis is hypothesized as a therapeutic target for obesity and some metabolic diseases, however, little data is available regarding the effects of medications on the gut mycobiome and associated host-bacterial-fungi interactions. Metformin is globally the most prescribed drug for type 2 diabetes mellitus (T2D), and its therapeutic effects cause decreased hepatic gluconeogenesis and consequently circulating blood glucose. Metformin treatment also impacts the gut microbiome composition, lowering circulating lipopolysaccharide and therefore reducing toll-like-receptor-mediated inflammation. To date, the potential effects of metformin on the gut mycobiome are entirely unknown. Therefore, this study aims to address this knowledge gap by characterizing the impact of metformin on the gut mycobiome of healthy adults and adults with pre-diabetes and T2D, defined by fasting plasma glucose values. We searched for published research articles with archived, publicly accessible shotgun metagenomics data and re-processed the data with one uniform bioinformatics pipeline to identify fungal taxa and relative abundance. Our analysis involved a total of 4510 samples from 13 individual studies ranging from randomized clinical trials to cross-sectional cohorts. We performed analyses on the compiled dataset to (1) characterize the impact of metformin treatment in healthy, pre-diabetic, and T2D individuals on the gut mycobiome; and (2) correlate the changes in relative abundance of fungal taxa to available clinical covariates, such as body mass index and fasting blood glucose. Secondary analysis of a randomized controlled trial of 44 newly diagnosed, treatment-naïve T2D patients who were prescribed metformin for three days demonstrated a significant effect of metformin on the gut mycobiome richness (Shannon and Simpson indices; t test p = 0.02 and p = 0.0061 respectively) and diversity (PERMANOVA p = 0.001). Additionally, the relative abundance of Saccharomyces,a well-characterized yeast genus, was significantly higher after three days of metformin treatment (Wilcoxon p = 0.019). This study provides novel insight into the interactions between human hosts, the gut microbiome, and the gut mycobiome in response to metformin, a widely used drug to treat T2D. Furthermore, these analyses may provide greater understanding of the role of the mycobiome-microbiome interaction in metabolic diseases.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
qsy发布了新的文献求助10
刚刚
冬虫夏草发布了新的文献求助10
1秒前
大虎发布了新的文献求助10
2秒前
打打应助正直的发卡采纳,获得10
2秒前
SciGPT应助Skyler666采纳,获得50
2秒前
Singularity应助yuaner采纳,获得10
2秒前
今后应助科研通管家采纳,获得10
3秒前
Starwalker应助科研通管家采纳,获得20
3秒前
天天快乐应助科研通管家采纳,获得10
4秒前
传奇3应助科研通管家采纳,获得10
4秒前
Kevin应助科研通管家采纳,获得10
4秒前
4秒前
4秒前
抱住仙人球应助WUYANG采纳,获得10
5秒前
田様应助xx采纳,获得10
10秒前
ZJHYNL应助九点一定起采纳,获得10
11秒前
14秒前
bluekids完成签到,获得积分10
17秒前
17秒前
17秒前
打打应助三毛采纳,获得10
18秒前
18秒前
mingyue应助神勇葵阴采纳,获得10
18秒前
鲁轶祎发布了新的文献求助10
18秒前
科研文献搬运工应助xmckkai采纳,获得30
19秒前
小蘑菇应助lewisll采纳,获得10
19秒前
19秒前
夏来应助nenoaowu采纳,获得20
21秒前
动听千山发布了新的文献求助30
22秒前
辛勤的乌完成签到,获得积分10
22秒前
24秒前
樊珩发布了新的文献求助10
24秒前
suodeheng完成签到,获得积分10
25秒前
kk发布了新的文献求助10
26秒前
26秒前
26秒前
不吃辣活不了完成签到,获得积分10
28秒前
28秒前
九秋霜发布了新的文献求助10
30秒前
meng136281发布了新的文献求助10
30秒前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Cognitive linguistics critical concepts in linguistics 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
氟盐冷却高温堆非能动余热排出性能及安全分析研究 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3050909
求助须知:如何正确求助?哪些是违规求助? 2708236
关于积分的说明 7412010
捐赠科研通 2352411
什么是DOI,文献DOI怎么找? 1245174
科研通“疑难数据库(出版商)”最低求助积分说明 605463
版权声明 595796