乌拉3
可选择标记
生物
毕赤酵母
遗传学
基因
营养不良
酿酒酵母
穿梭机载体
质粒
重组DNA
突变体
载体(分子生物学)
作者
G P Cereghino,Joan Lin Cereghino,Anthony Jay Sunga,Monique Johnson,May Lim,Martina A.G. Gleeson,James M. Cregg
出处
期刊:Gene
[Elsevier]
日期:2001-01-01
卷期号:263 (1-2): 159-169
被引量:103
标识
DOI:10.1016/s0378-1119(00)00576-x
摘要
We describe the isolation and characterization of three new biosynthetic genes-ARG4, ADE1, and URA3-from the methylotrophic yeast Pichia pastoris. The predicted products of the genes share significant sequence similarity to their Saccharomyces cerevisiae counterparts, namely argininosuccinate lyase, PR-aminoimidazolesuccinocarboxamide synthase, and orotidine-5'-phosphate decarboxylase, respectively. Along with the previously described HIS4 gene, each gene was incorporated as the yeast selectable marker into a set of shuttle vectors designed to express foreign genes in P. pastoris. In addition, we have constructed a series of host strains containing all possible combinations of ade1, arg4, his4, and ura3 auxotrophies to be used with these new vectors.
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