白细胞介素10
白细胞介素4
流式细胞术
细胞因子
免疫学
医学
白细胞介素17
免疫系统
转化生长因子
内科学
作者
Poonam Gaur,Avadhesh Kumar Singh,Nootan Kumar Shukla,Srinibas Das
标识
DOI:10.1016/j.humimm.2014.01.011
摘要
Altered cytokine production can lead to immune dysfunction in cancer patients. Hence, we investigated the cytokine balance in oral squamous cell carcinoma (OSCC) patients and their significance in providing new therapeutic insights. We quantified Th17 (IL17A), Treg (TGFβ1), Th1 (IL2, IFNγ) and Th2 (IL4, IL10) like cytokines in the sera of 78 cases and 39 controls by ELISA. The intracellular expression of these cytokines was analyzed in 10 subjects from each group by flow cytometry. Serum levels of IL17A, TGFβ1, IL4 and IL10 were significantly higher while IL2 and IFNγ were relatively lower in patients as compared to controls. TGFβ1 (r = 0.55), IL4 (r = 0.75) and IL10 (r = 0.80) significantly (P < 0.0001) correlated with disease progression and their elevated levels showed increased odd ratios of approximately 18, 14 and 37, respectively. IL17A appeared as a risk factor (OR = 2.21, 95% CI = 0.89–5.42) although statistically insignificant. The levels neither correlated with disease progression nor with TGFβ1, IL4 and IL10 but showed positive association with IL2 (r = 0.51, P < 0.0001) and IFNγ (r = 0.24). Flow cytometry data also showed similar trend. We reported a distinct TGFβ1 and Th2 (IL4, IL10) polarization with a borderline elevation of IL17A while, a suppression of Th1 (IL2, IFNγ) cytokines in OSCC patients.
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