蜂胶
生物膜
微生物学
MTT法
福尔马赞
肉汤微量稀释
化学
传统医学
结晶紫
食品科学
细胞生长
生物
抗生素
细菌
最小抑制浓度
医学
生物化学
遗传学
作者
Bochra Kouidhi,Tarek Zmantar,Amina Bakhrouf
出处
期刊:Anaerobe
[Elsevier]
日期:2010-12-01
卷期号:16 (6): 566-571
被引量:80
标识
DOI:10.1016/j.anaerobe.2010.09.005
摘要
Propolis is a multifunctional substance used by bees to maintain the safety of their hives. It is worldwide used for its potential therapeutic effects. In this study, Tunisian propolis ethanol extract (EEP) was tested for their anti-cariogenic, anti-biofilms and antiproliferative effects of many cell lines. The Tunisian EEP was evaluated in vitro against 33 oral pathogens including streptococci and enterococci using broth microdilution method. The anti-biofilms activity of EEP was assessed via Crystal Violet staining and MTT assays. The Tunisian EEP antiproliferative effect was evaluated on normal (MRC-5) and cancer cell lines (HT-29, A549, Hep-2, raw 264.7, Vero) by the ability of the cells to metabolically reduce MTT to a formazan dye. Our results revealed that Tunisian EEP possessed excellent protective effects against cariogenic and biofilms activity of oral streptococci. Furthermore, EEP showed a strong antiproliferative potencies against all studied cancer cell lines as judged by IC50 and its value ranges from 15.7 ± 3.4 to 200 ± 22.2 μg mL⁻¹. These results suggest that EEP is able to inhibit cancer cell proliferation, cariogenic bacteria and oral biofilms formation. It could have a promising role in the future medicine and nutrition when used as antibiotic or food additive.
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