丙烯酸
聚(N-异丙基丙烯酰胺)
血红蛋白
化学
高分子化学
分离(微生物学)
自愈水凝胶
生物化学
有机化学
聚合物
共聚物
生物
生物信息学
作者
Xuwei Chen,Shuai Chen,Jianhua Wang
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2010-01-01
卷期号:135 (7): 1736-1736
被引量:15
摘要
A pH-responsive poly(N-isopropylacrylamide-co-acrylic acid) hydrogel was prepared by an aqueous dispersion polymerization process. The number of carboxylic groups distributed on the surface of the hydrogel was found to be 1.89 mmol g−1. The hydrogel exhibits excellent binding selectivity to the protein species of interest within certain pH regions. In the present work, it was applied to the selective isolation of hemoglobin from human whole blood. Some important experimental parameters governing the sorption property, including the pH of sample solution, the ionic strength and sorption time, were investigated. Using 2.0 mg of the hydrogel as a sorption medium and suitable acidity of pH 6.0, a sorption efficiency of 93% was achieved for 200 μg mL−1 of hemoglobin in 1.0 mL of sample solution. Afterwards, the absorbed hemoglobin could be readily recovered using 1.0 mL of Tris-HCl buffer (pH 8.6, 0.01 mol L−1), giving rise to recovery of ca. 76%. The practical applicability of this system was further demonstrated by processing human whole blood for the isolation of hemoglobin, and satisfactory results were obtained by assay with SDS-PAGE.
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