碳纳米管
光致发光
自体荧光
材料科学
量子产额
聚乙二醇
纳米技术
荧光
红外线的
光电子学
荧光寿命成像显微镜
化学
光学
有机化学
物理
作者
Kevin Welsher,Zhuang Liu,Sarah P. Sherlock,Joshua T. Robinson,Zhuo Chen,Dan Daranciang,Hongjie Dai
标识
DOI:10.1038/nnano.2009.294
摘要
The near-infrared photoluminescence intrinsic to semiconducting single-walled carbon nanotubes is ideal for biological imaging owing to the low autofluorescence and deep tissue penetration in the near-infrared region beyond 1 µm. However, biocompatible single-walled carbon nanotubes with high quantum yield have been elusive. Here, we show that sonicating single-walled carbon nanotubes with sodium cholate, followed by surfactant exchange to form phospholipid–polyethylene glycol coated nanotubes, produces in vivo imaging agents that are both bright and biocompatible. The exchange procedure is better than directly sonicating the tubes with the phospholipid–polyethylene glycol, because it results in less damage to the nanotubes and improves the quantum yield. We show whole-animal in vivo imaging using an InGaAs camera in the 1–1.7 µm spectral range by detecting the intrinsic near-infrared photoluminescence of the ‘exchange’ single-walled carbon nanotubes at a low dose (17 mg l−1 injected dose). The deep tissue penetration and low autofluorescence background allowed high-resolution intravital microscopy imaging of tumour vessels beneath thick skin. Single-walled carbon nanotubes can be modified into bright and biocompatible agents for high resolution whole-animal imaging at wavelengths in the 1100–1700 nm region.
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