To Enhance the Production of Human-Like Collagen II by Amino Acid Addition Using Response Surface Methodology

Amino acids, the basic unit of protein molecules, are closely related with biological activities. In order to enhance the expression of human-like collagen (HLC), amino acids were added into fermentation broth, and response surface methodology (RSM) to optimize the categories and the concentration of amino acids. The results of PB-design showed that only the dosage of alanine and glutamate had significant effects on the production of HLC. Quadratic polynomial models were established after analyzing the results of CCD-design. The optimal concentration of alanine and glutamate were 3.78gL-1、and 2.52g•L-1 respectively. Under the optimal cultivation condition the maximal production of human-like collagen could be up to 0.292g•L-1 which was 11.45% higher than that of the control group 0.262g•L-1 (the highest production of the former study).