大肠杆菌
肌钙蛋白复合物
肌钙蛋白
肌钙蛋白I
蛋白质亚单位
变构调节
肌钙蛋白T
肌钙蛋白C
生物化学
化学
生物
心脏病学
医学
酶
基因
心肌梗塞
标识
DOI:10.1016/j.pep.2012.10.005
摘要
Cardiomyopathy-related mutations in human cardiac troponin subunits, including troponin C (hcTnC), troponin I (hcTnI), and troponin T (hcTnT), are well-documented. Recently, it has been recognised that human cardiac troponin (hcTn) is a sophisticated allosteric system. Therefore, the effect of drugs on this protein complex should be studied with assembled hcTn rather than a short fragment of a subunit or the subunit itself. Here, we describe the expression and assembly of active hcTn in Escherichia coli, a novel method that is rapid and simple, and produces large amounts of functional hcTn.
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