去细胞化
灌注
心力衰竭
心脏病学
心功能曲线
生物医学工程
移植
医学
内科学
细胞外基质
心脏移植
组织工程
化学
生物化学
作者
Harald C. Ott,T Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Théoden I. Netoff,Doris A. Taylor
出处
期刊:Nature Medicine
[Springer Nature]
日期:2008-01-13
卷期号:14 (2): 213-221
被引量:2502
摘要
About 3,000 individuals in the United States are awaiting a donor heart; worldwide, 22 million individuals are living with heart failure. A bioartificial heart is a theoretical alternative to transplantation or mechanical left ventricular support. Generating a bioartificial heart requires engineering of cardiac architecture, appropriate cellular constituents and pump function. We decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent acellular valves and intact chamber geometry. To mimic cardiac cell composition, we reseeded these constructs with cardiac or endothelial cells. To establish function, we maintained eight constructs for up to 28 d by coronary perfusion in a bioreactor that simulated cardiac physiology. By day 4, we observed macroscopic contractions. By day 8, under physiological load and electrical stimulation, constructs could generate pump function (equivalent to about 2% of adult or 25% of 16-week fetal heart function) in a modified working heart preparation.
科研通智能强力驱动
Strongly Powered by AbleSci AI